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time qpcr plates  (Eppendorf AG)


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    Eppendorf AG time qpcr plates
    Time Qpcr Plates, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 99/100, based on 201118 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/time+qpcr+plates/Eppendorf/pmc12934228-261-20-22
    Average 99 stars, based on 201118 article reviews
    time qpcr plates - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    SYBR Green Assay:

    Article Title: N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation
    Article Snippet: The cDNA was diluted 10× with RNAse-free water (Sigma) and stored at −20°C until further use. .. The cDNA was mixed with forward and reverse primers ( ) and iTaq universal SYBR Green Supermix (Biorad) in real time-qPCR plates (Eppendorf)., , , , , Amplification was performed using the CFX connect Real-time PCR machine (Biorad) with the following cycling program: initial denaturation at 95°C for 3 min, followed by 40 cycles of 10 s at 95°C and 30 s at 60°C. ..

    Amplification:

    Article Title: N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation
    Article Snippet: The cDNA was diluted 10× with RNAse-free water (Sigma) and stored at −20°C until further use. .. The cDNA was mixed with forward and reverse primers ( ) and iTaq universal SYBR Green Supermix (Biorad) in real time-qPCR plates (Eppendorf)., , , , , Amplification was performed using the CFX connect Real-time PCR machine (Biorad) with the following cycling program: initial denaturation at 95°C for 3 min, followed by 40 cycles of 10 s at 95°C and 30 s at 60°C. ..

    Real-time Polymerase Chain Reaction:

    Article Title: N1MΨU-modified mRNA vaccines break the mold in fish by enhancing innate immune activation
    Article Snippet: The cDNA was diluted 10× with RNAse-free water (Sigma) and stored at −20°C until further use. .. The cDNA was mixed with forward and reverse primers ( ) and iTaq universal SYBR Green Supermix (Biorad) in real time-qPCR plates (Eppendorf)., , , , , Amplification was performed using the CFX connect Real-time PCR machine (Biorad) with the following cycling program: initial denaturation at 95°C for 3 min, followed by 40 cycles of 10 s at 95°C and 30 s at 60°C. ..



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    A) Relative expression of Schwann cell marker genes in DFT1_4906 (blue) and DFT2_RV (red) cells as measured by qPCR. Gene expression was quantified by the relative standard curve method, using RPL13A as a housekeeping control gene. Error bars are SEM. Significance of differential expression as determined by T-test is indicated by stars (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001). B) Two-way ANOVA analysis of the response of DFT1 and DFT2 cells to IFNγ at Schwann cell markers, indicating the significance p-values of differential expression between cell lines, treatments and the interaction between the two. A P < 0.05 indicates a significant result, denoted by bold red text. C-D) Relative expression of Schwann cell marker genes as measured by qRT-PCR in C) DFT1_4906 and D) DFT2_RV cells with and without IFNγ treatment. Gene expression was quantified by the relative standard curve method, using RPL13A as a housekeeping control gene. Error bars are SEM. Note that PDGFRA was not detected in DFT1 cells and MAG was not detected in DFT2 cells. Significance of differential expression as determined by T-test is indicated by stars (* = P < 0.05, ** = P < 0.01). qPCR data used to generate Fig 2 and can be found in .

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    Figure Lengend Snippet: A) Relative expression of Schwann cell marker genes in DFT1_4906 (blue) and DFT2_RV (red) cells as measured by qPCR. Gene expression was quantified by the relative standard curve method, using RPL13A as a housekeeping control gene. Error bars are SEM. Significance of differential expression as determined by T-test is indicated by stars (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001). B) Two-way ANOVA analysis of the response of DFT1 and DFT2 cells to IFNγ at Schwann cell markers, indicating the significance p-values of differential expression between cell lines, treatments and the interaction between the two. A P < 0.05 indicates a significant result, denoted by bold red text. C-D) Relative expression of Schwann cell marker genes as measured by qRT-PCR in C) DFT1_4906 and D) DFT2_RV cells with and without IFNγ treatment. Gene expression was quantified by the relative standard curve method, using RPL13A as a housekeeping control gene. Error bars are SEM. Note that PDGFRA was not detected in DFT1 cells and MAG was not detected in DFT2 cells. Significance of differential expression as determined by T-test is indicated by stars (* = P < 0.05, ** = P < 0.01). qPCR data used to generate Fig 2 and can be found in .

    Article Snippet: A full list of primers and conditions can be found in at https://doi.org/10.5258/SOTON/D1975 . qRT-PCR was performed using 250 ng of cDNA, 0.25 μM of forward and reverse primers and 1X PrecisionPlus qPCR Master Mix with SYBR green and ROX (PrimerDesign, PrecisionPLUS-R-SY) on a BrightWhite real-time PCR plastic qPCR plate (PrimerDesign, BW-FAST). qRT-PCR experiments were run on a StepOne Plus real-time PCR system (Applied Biosciences) on a standard protocol with a melt curve to assess amplicon purity.

    Techniques: Expressing, Marker, Quantitative RT-PCR