Journal: PLoS Pathogens
Article Title: The differentiation state of the Schwann cell progenitor drives phenotypic variation between two contagious cancers
doi: 10.1371/journal.ppat.1010033
Figure Lengend Snippet: A) Relative expression of Schwann cell marker genes in DFT1_4906 (blue) and DFT2_RV (red) cells as measured by qPCR. Gene expression was quantified by the relative standard curve method, using RPL13A as a housekeeping control gene. Error bars are SEM. Significance of differential expression as determined by T-test is indicated by stars (* = P < 0.05, ** = P < 0.01, *** = P < 0.001, **** = P < 0.0001). B) Two-way ANOVA analysis of the response of DFT1 and DFT2 cells to IFNγ at Schwann cell markers, indicating the significance p-values of differential expression between cell lines, treatments and the interaction between the two. A P < 0.05 indicates a significant result, denoted by bold red text. C-D) Relative expression of Schwann cell marker genes as measured by qRT-PCR in C) DFT1_4906 and D) DFT2_RV cells with and without IFNγ treatment. Gene expression was quantified by the relative standard curve method, using RPL13A as a housekeeping control gene. Error bars are SEM. Note that PDGFRA was not detected in DFT1 cells and MAG was not detected in DFT2 cells. Significance of differential expression as determined by T-test is indicated by stars (* = P < 0.05, ** = P < 0.01). qPCR data used to generate Fig 2 and can be found in .
Article Snippet: A full list of primers and conditions can be found in at https://doi.org/10.5258/SOTON/D1975 . qRT-PCR was performed using 250 ng of cDNA, 0.25 μM of forward and reverse primers and 1X PrecisionPlus qPCR Master Mix with SYBR green and ROX (PrimerDesign, PrecisionPLUS-R-SY) on a BrightWhite real-time PCR plastic qPCR plate (PrimerDesign, BW-FAST). qRT-PCR experiments were run on a StepOne Plus real-time PCR system (Applied Biosciences) on a standard protocol with a melt curve to assess amplicon purity.
Techniques: Expressing, Marker, Quantitative RT-PCR
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